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PNNL Publications 2020 Smith J.N., K.J. Tyrrell, J.P. Smith, J.P. Smith, K.K. Weitz, and W.D. Faber. 2020. "Linking Internal Dosimetries of the Propyl Metabolic Series in Rats and Humans Using Physiologically Based Pharmacokinetic (PBPK) Modeling." Regulatory Toxicology and Pharmacology 110. PNNL...

The Illumina MiSeq System Sequencer is a high-throughput DNA sequencer machine developed and manufactured by Illumina, and is designed for sequencing data acquisition using synthesis technology to provide an end-to-end solution (cluster generation, amplification, sequencing, and data analysis) in a...

LIQUID Software Overview LIQUID provides users with the capability to process high throughput data and contains a customizable target library and scoring model per project needs. The graphical user interface provides visualization of multiple lines of spectral evidence for each lipid identification...

Model Soil Consortium 2 (MSC-2) Bacterial Isolate Genomes Model Soil Consortium 2 (MSC-2) bacterial isolate genome collections are derived from the soil consortium "MSC-1" multi-species cultured isolate genome collections from a previously reported WA-IsoC_MSC1.1.0 collection of a naturally evolved...

16s data from MSC-2 growth 3 fastq of 16s amplicon data of MSC2 1 csv file of raw ASV abundances for MSC2

PerCon SFA Project Data Catalog Collection (uploads pending) The Persistence Control of Engineered Functions in Complex Soil Microbiomes Project (PerCon SFA), at Pacific Northwest National Laboratory (PNNL), is a Genomic Sciences Program Biosystems Design Research Science Focus Area project. The...

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"Visualizing the Hidden Half: Plant-Microbe Interactions in the Rhizosphere" Plant roots and the associated rhizosphere constitute a dynamic environment that fosters numerous intra- and interkingdom interactions, including metabolite exchange between plants and soil mediated by root exudates and the...

Pending Review Microbiomes contribute to multiple ecosystem services by transforming organic matter in soil. Extreme shifts in the environment, such as drying-rewetting cycles during drought, can impact microbial metabolism of organic matter by altering their physiology and function. These...

Multi-Omics Extended Data Package DOI Rapid Remodeling of the Soil Lipidome in Response to a Drying-Rewetting Event This study reveals specific changes in lipids and metabolites that are indicative of stress adaptation, substrate use, and cellular recovery during soil drying and subsequent rewetting...

MPLEx Universal Protocol for Single-Sample Integrative Proteomic, Metabolomic, and Lipidomic Analyses Mass Spectrometry Experimental Protocol (SIO:001043) | Standard Operating Procedure (SIO:000964) The Metabolite, Protein and Lipid Extraction (MPLEx) method protocol is a robust and applicable...

"DNA Viral Diversity, Abundance, and Functional Potential Vary across Grassland Soils with a Range of Historical Moisture Regimes" Soil viruses are abundant, but the influence of the environment and climate on soil viruses remains poorly understood. Here, we addressed this gap by comparing the...

"Deconstructing the Soil Microbiome into Reduced-Complexity Functional Modules" The soil microbiome represents one of the most complex microbial communities on the planet, encompassing thousands of taxa and metabolic pathways, rendering holistic analyses computationally intensive and difficult. Here...

This data set provides the 16S microbial community composition via DNA sequence analysis from ingrowth peat and sand cores at the South End bog in 2013. These samples were collected outside the experimental enclosures and are pre-treatment with no experimental manipulation. These are part of the...

This data set provides ITS fungal community composition via DNA and cDNA sequence analysis at the time of peat coring for Deep Peat Heating (DPH) and Whole Ecosystem Warming (WEW) for 2014-2017 from the Spruce and Peatlands Under Changing Environments (SPRUCE). Samples were extracted using a Qiagen...

This data set provides the ITS fungal community composition via DNA and cDNA sequence analysis for peat and sand ingrowth cores collected before and during Deep Peat Heating (DPH) and Whole Ecosystem Warming (WEW) for 2015-2016 from the Spruce and Peatlands Under Changing Environments (SPRUCE)...