The Phenotypic Response of the Soil Microbiome to Environmental Perturbations Project (Soil Microbiome) at Pacific Northwest National Laboratory is a Genomic Sciences Program Science Focus Area (SFA) Project operating under the Environmental Microbiome Science Research Area. The Soil Microbiome SFA project aims to develop a systems-level understanding of soil microbial and phenotypic responses to changing moisture through a tractable, spatially explicit examination of the molecular and ecological interactions occurring within and between microbial consortia in soil. PNNL is performing integrated experiments to confront both the scaling challenges and interkingdom interactions that regulate networks of biochemical reactions. High-resolution experimental data are being used to parameterize agent-based models. Genome-enabled predictions of spatially explicit microbial interactions are being validated in reduced-complexity microbial consortia using advanced chemical imaging and other techniques unique to PNNL.
PNNL’s Soil Microbiome SFA project repository on DataHub allows for exploring and downloading integrated experimental omics data, experimental metadata, pre- and post-processed data files, and other associated materials directly related to experimental project publication data. For updates and more information, visit the PNNL Soil Microbiome Science Focus Area Project homepage.
- Sponsor Page: Phenotypic Response of Soil Microbiomes (GSP)
- Main Project Homepage: Phenotypic Response of the Soil Microbiome to Environmental Perturbations (PNNL)
- Project Data Repository: https://doi.org/10.25584/PRJ.SFA/1780765 (PNNL DataHub)
- KBase Collaboration: Metabolic network reconstruction of microorganisms and communities
This research is supported in part by the U.S. Department of Energy (DOE), Office of Biological and Environmental Research (BER), as part of BER’s Genomic Science Program (GSP, https://genomicscience.energy.gov) and is a contribution of the Pacific Northwest National Laboratory (PNNL) Secure Biosystems Design Science Focus Area "Phenotypic Response of the Soil Microbiome to Environmental Perturbations Project ". A portion of this work was performed under the EMSL user project award 10.46936/cpcy.proj.2021.60161/60000437, utilizing instrument capabilities operating at the Environmental Molecular Sciences Laboratory, a DOE Office of Science User Facility sponsored by the Biological and Environmental Research program under Contract No. DE-AC05-76RL01830.
- Biological Sciences Division, Earth and Biological Sciences Directorate, Pacific Northwest National Laboratory, Richland, Washington 99352, USA.
- Environmental Molecular Sciences Division, Earth and Biological Sciences Directorate, Pacific Northwest National Laboratory, Richland, Washington 99352, USA. (EMSL User Facility)
- School of Biological Sciences, Washington State University, Pullman, Washington 99163, USA.
- Department of Ecology, Evolution and Organismal Biology, Iowa State University, Ames, Iowa 50011, USA.
- Division of Biology, Kansas State University, Manhattan, Kansas, USA.
- Department of Biological Systems Engineering, University of Nebraska-Lincoln, Lincoln, Nebraska 68588, USA.
DataHub CRediT (Contributor Roles Taxonomy)
- LN Anderson (0000-0002-8741-7823) - RDM Data Packaging (Data curation, Validation, Writing – review & editing, Visualization)
- M Pleake (0000-0002-7807-8364) - IT Engineering RDM Support (Resources)